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cell culture human aortic smc haosmc  (ATCC)


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    ATCC cell culture human aortic smc haosmc
    Cell Culture Human Aortic Smc Haosmc, supplied by ATCC, used in various techniques. Bioz Stars score: 95/100, based on 388 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+aortic+smc+haosmc/Primary+Aortic+Smooth+Muscle+Cells%3B+Normal%2C+Human/pm41672970-321-0-9
    Average 95 stars, based on 388 article reviews
    cell culture human aortic smc haosmc - by Bioz Stars, 2026-09
    95/100 stars

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    Cell Culture:

    Article Title: Inhibition of MLKL impairs abdominal aortic aneurysm development by attenuating smooth muscle cell necroptosis
    Article Snippet: Doxycycline Hydrochloride as purchased from Sigma (Cat. No. D3072-1ML). .. Human aortic SMC (HAoSMC) were purchased from ATCC (PCS-100-012, ATCC) and propagated in Vascular Cell Basal Medium (PCS-100-030, ATCC) supplemented with Vascular Smooth Muscle Cell Growth Kit (PCS-100-042, ATCC) as per the manufactures protocol in standard cell culture incubator at 5% CO 2 , 37 °C. .. HL-60 cells were maintained in RPMI-1640 (72400047, Gibco) with 10% FBS (F9665, Sigma), 1x GlutaMAXTM Supplement (35050061, Gibco), 1x Penicillin-streptomycin (15140122, Gibco) in a 5% CO 2 atmosphere at 37 °C.

    Article Title: Loss of MLKL impairs abdominal aortic aneurysm development by attenuating smooth muscle cell necroptosis
    Article Snippet: Doxycycline Hydrochloride as purchased from Sigma (Cat. No. D3072-1ML). .. Human aortic SMC (HAoSMC) were purchased from ATCC (PCS-100-012, ATCC) and propagated in Vascular Cell Basal Medium (PCS-100-030, ATCC) supplemented with Vascular Smooth Muscle Cell Growth Kit (PCS-100-042, ATCC) as per the manufacturer's protocol in a standard cell culture incubator at 5% CO 2 , 37 °C. .. HL-60 cells were maintained in RPMI-1640 (72400047, Gibco) with 10% FBS (F9665, Sigma), 1x GlutaMAXTM Supplement (35050061, Gibco), 1x Penicillin-streptomycin (15140122, Gibco) in a 5% CO 2 atmosphere at 37 °C.



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    LIVE/DEAD stain of <t>SMCs</t> cocultured with Silk MPs and SIMPs incubated in PBS or digested with Protease XIV showed low cytotoxicity of the particles. SMCs were <t>also</t> <t>cultured</t> with water as a negative control. Green channels (a,c,e, and g) showed confluent live cells in all MP treated groups compared to the negative control (i). Red channels (b,d,f, and h) showed minimal amounts of red (dead) cells compared to the negative control (j). The number of live (green) and dead cells (red) was counted across treatments ( n = 3), and the percentage of live and dead cells was compared to the negative control. There was a significantly higher percentage of live cells (k) and simultaneously a significantly lower percentage of dead cells (l) in the MP groups compared to the negative control. Scale bar = 100 μm ** = p < 0.01, *** = p < 0.001
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    LIVE/DEAD stain of SMCs cocultured with Silk MPs and SIMPs incubated in PBS or digested with Protease XIV showed low cytotoxicity of the particles. SMCs were also cultured with water as a negative control. Green channels (a,c,e, and g) showed confluent live cells in all MP treated groups compared to the negative control (i). Red channels (b,d,f, and h) showed minimal amounts of red (dead) cells compared to the negative control (j). The number of live (green) and dead cells (red) was counted across treatments ( n = 3), and the percentage of live and dead cells was compared to the negative control. There was a significantly higher percentage of live cells (k) and simultaneously a significantly lower percentage of dead cells (l) in the MP groups compared to the negative control. Scale bar = 100 μm ** = p < 0.01, *** = p < 0.001

    Journal: ACS Applied Materials & Interfaces

    Article Title: Chemical Conjugation of Iron Oxide Nanoparticles for the Development of Magnetically Directable Silk Particles

    doi: 10.1021/acsami.4c17536

    Figure Lengend Snippet: LIVE/DEAD stain of SMCs cocultured with Silk MPs and SIMPs incubated in PBS or digested with Protease XIV showed low cytotoxicity of the particles. SMCs were also cultured with water as a negative control. Green channels (a,c,e, and g) showed confluent live cells in all MP treated groups compared to the negative control (i). Red channels (b,d,f, and h) showed minimal amounts of red (dead) cells compared to the negative control (j). The number of live (green) and dead cells (red) was counted across treatments ( n = 3), and the percentage of live and dead cells was compared to the negative control. There was a significantly higher percentage of live cells (k) and simultaneously a significantly lower percentage of dead cells (l) in the MP groups compared to the negative control. Scale bar = 100 μm ** = p < 0.01, *** = p < 0.001

    Article Snippet: Human aortic vascular smooth muscle cells (SMCs) from Cell Applications (354–05a, San Diego, CA) were cultured at 37 °C and 5% CO 2 (3530, Isotemp, ThermoFisher Scientific) in supplemented basal media (311 K-500, Cell Applications Inc.) between passages 6–10.

    Techniques: Staining, Incubation, Cell Culture, Negative Control