Journal: ACS Applied Materials & Interfaces
Article Title: Chemical Conjugation of Iron Oxide Nanoparticles for the Development of Magnetically Directable Silk Particles
doi: 10.1021/acsami.4c17536
Figure Lengend Snippet: LIVE/DEAD stain of SMCs cocultured with Silk MPs and SIMPs incubated in PBS or digested with Protease XIV showed low cytotoxicity of the particles. SMCs were also cultured with water as a negative control. Green channels (a,c,e, and g) showed confluent live cells in all MP treated groups compared to the negative control (i). Red channels (b,d,f, and h) showed minimal amounts of red (dead) cells compared to the negative control (j). The number of live (green) and dead cells (red) was counted across treatments ( n = 3), and the percentage of live and dead cells was compared to the negative control. There was a significantly higher percentage of live cells (k) and simultaneously a significantly lower percentage of dead cells (l) in the MP groups compared to the negative control. Scale bar = 100 μm ** = p < 0.01, *** = p < 0.001
Article Snippet: Human aortic vascular smooth muscle cells (SMCs) from Cell Applications (354–05a, San Diego, CA) were cultured at 37 °C and 5% CO 2 (3530, Isotemp, ThermoFisher Scientific) in supplemented basal media (311 K-500, Cell Applications Inc.) between passages 6–10.
Techniques: Staining, Incubation, Cell Culture, Negative Control